Journal: Cell stem cell

Article Title: Histone Acetyltransferase MOF Blocks Acquisition of Quiescence in Ground-State ESCs through Activating Fatty Acid Oxidation

doi: 10.1016/j.stem.2020.06.005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The primary and secondary antibodies included: Anti-β-ACTIN (Santa Cruz Biotechnology, #sc47778, 1:2000), Mouse anti-OCT3/4 (Santa Cruz Biotechnology, #sc-5279, 1:100), Mouse anti-KLF4 (Santa Cruz Biotechnology, #sc-166238, 1:100), Goat anti-TFCP2L1 (R & D Systems, #AF5726, 1:100), Rabbit anti-MOF (Bethyl Laboratories, #A300-992A, 1:1000), Rabbit anti-H4K16ac (Millipore, #07-329, 1:2000), Rabbit anti-H3K27ac (Abcam, #ab4729, 1:1000), Rabbit anti-H3K9ac (Abcam, #ab4441, 1:1000), Rabbit anti-Histone H3 (Abcam, #ab1791, 1:2000), Rabbit anti-Histone H4 (Active Motif, #39269, 1:2000), Rabbit anti-H4ac (Millipore, #06-866, 1:2000), Rabbit anti-H3K56ac (Abcam, #ab76307, 1:2000), anti-mouse IgG Horseradish Peroxidase-linked whole antibody from Sheep (GE Healthcare, #NX931V, 1:3000), anti-Rabbit IgG Horseradish Peroxidase-linked whole antibody (GE Healthcare, #NA934, 1:3000) and Donkey anti-Goat IgG H&L HRP (Abcam, #ab6885, 1:2000).

Techniques: Recombinant, Plasmid Preparation, Flow Cytometry, RNA HS Assay, Software, Inhibition